【佳學基因檢測】人參皂甙抑制舌癌細胞遷移和侵襲所需要的基因檢測結果

1年靶向藥物要多少錢評價

探索明白《J Appl Oral Sci》在. 2022 Sep 2;30:e20220144.發(fā)表了一篇題目為《人參皂甙Rd通過H19/miR-675-5p/CDH1軸抑制舌癌細胞遷移和侵襲》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Lu Chang, Dongxu Wang, Shaoning Kan, Ming Hao, Huimin Liu, Zhijing Yang, Qianyun Xia, Weiwei Liu 等完成。促進了腫瘤的正確治療與個性化用藥的發(fā)展，進一步強調了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內容關鍵詞：

人參皂甙,miR-675-5p,CDH1,抑制,舌癌細胞,遷移,侵襲,基因檢測結果

腫瘤靶向治療基因檢測臨床應用結果

舌鱗狀細胞癌轉移抑制基因檢測研究目的：舌鱗狀細胞癌（TSCC）是一種口腔癌，惡性程度高，早期遷移和侵襲頻繁。只有少數(shù)藥物可以治療舌癌。 Ginsen總生存期ide Rd 是一種具有抗癌作用的人參提取物。許多非編碼RNA在舌癌中異常表達，從而影響其發(fā)生和發(fā)展。 H19和miR-675-5p可以促進癌細胞生長。本研究旨在分析人參皂甙Rd對舌癌H19和miR-675-5p的調控作用。舌鱗狀細胞癌轉移抑制基因檢測研究方法：采用CCK8和流式細胞術研究其生長和凋亡。 Transwell測定用于評估侵襲；評估遷移的傷口愈合試驗；和集落形成測定以測試細胞形成集落的能力。通過 qPCR 分析 H19、miR-675-5p 和 CDH1 表達。使用蛋白質印跡檢測 E-鈣粘蛋白表達。采用CRISPR/cas9系統(tǒng)敲除CDH1。舌鱗狀細胞癌轉移抑制基因檢測研究結果：人參皂甙Rd抑制SCC9細胞生長，增加細胞凋亡。 Ginsen總生存期ide Rd 還抑制 SCC9 細胞的遷移和侵襲。 H19 和 miR-675-5p 高表達，而 CDH1 和 E-cadherin 表達低。 H19 和 miR-675-5p 促進 SCC9 轉移。相反，CDH1 和 E-cadherin 抑制 SCC9 細胞的轉移。生物信息學分析顯示miR-675-5p與CDH1相關。人參皂苷Rd處理后H19和miR-675-5p表達降低，而CDH1和E-cadherin表達增加。舌鱗狀細胞癌轉移抑制基因檢測研究結論：人參皂苷Rd通過H19/miR-675-5p/CDH1軸抑制舌癌細胞遷移和侵襲。

腫瘤發(fā)生與反復轉移國際數(shù)據(jù)庫描述：

Objective: Tongue squamous cell carcinoma (TSCC) is an oral cancer, with high malignancy and frequent early migration and invasion. Only a few drugs can treat tongue cancer. Ginsenoside Rd is a ginseng extract with anti-cancer effects. Many noncoding RNAs are abnormally expressed in tongue cancer, thus influencing its occurrence and development. H19 and miR-675-5p can promote cancer cell growth. This study aimed to analyze the regulation effect of ginsenoside Rd on H19 and miR-675-5p in tongue cancer.Methodology: We used CCK8 and flow cytometry to study the growth and apoptosis. Transwell assay was used to assess invasion; wound-healing assay to assess migration; and colony formation assays to test the ability of cells to form colonies. H19, miR-675-5p, and CDH1 expressions were analyzed by qPCR. E-cadherin expression was detected using western blot. CRISPR/cas9 system was used for CDH1 knockout.Results: Ginsenoside Rd inhibited the growth and increased the apoptosis of SCC9 cells. Ginsenoside Rd also inhibited the migration and invasion of SCC9 cells. H19 and miR-675-5p were highly expressed, while CDH1 and E-cadherin expressions were low. H19 and miR-675-5p promoted SCC9 metastasis. In contrast, CDH1 and E-cadherin inhibited the metastasis of SCC9 cells. Bioinformatics analysis showed that miR-675-5p was associated with CDH1. H19 and miR-675-5p expressions decreased after ginsenoside Rd treatment, while CDH1 and E-cadherin expressions increased.Conclusions: Ginsenoside Rd inhibits tongue cancer cell migration and invasion via the H19/miR-675-5p/CDH1 axis.