【佳學(xué)基因檢測(cè)】ALDH+ 肺腺癌干細(xì)胞中新型多組學(xué)表達(dá)圖譜的鑒定和基于圖譜的競(jìng)爭(zhēng)性內(nèi)源性 RNA 網(wǎng)絡(luò)的元分析

病原微生物基因檢測(cè)多少錢要點(diǎn)

體會(huì)癌的基因檢測(cè)基因解碼如何創(chuàng)新治療認(rèn)識(shí)到《Biomed Res Int》在.?2022 Aug 31;2022:9545609.發(fā)表了一篇題目為《ALDH+ 肺腺癌干細(xì)胞中新型多組學(xué)表達(dá)圖譜的鑒定和基于圖譜的競(jìng)爭(zhēng)性內(nèi)源性 RNA 網(wǎng)絡(luò)的元分析》腫瘤靶向藥物治療基因檢測(cè)臨床研究文章。該研究由Wei Yang,?Yong Liang,?Yuanyuan Zheng,?Haitao Luo,?Xiaofei Yang,?Furong Li等完成。促進(jìn)了腫瘤的正確治療與個(gè)性化用藥的發(fā)展，進(jìn)一步強(qiáng)調(diào)了基因信息檢測(cè)與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究?jī)?nèi)容關(guān)鍵詞：

腫瘤靶向治療基因檢測(cè)臨床應(yīng)用結(jié)果

ALDH+ H1975 肺腺癌干細(xì)胞 (LSC) 是在肺腺癌 (LUAD) 中發(fā)現(xiàn)的罕見細(xì)胞群。 LSCs 可以自我更新，驅(qū)動(dòng)腫瘤的發(fā)生、生長(zhǎng)、轉(zhuǎn)移和反復(fù)，并且由于其對(duì)藥物和化學(xué)療法的內(nèi)在抗性，也是預(yù)后不良的主要原因。因此，LSCs 是 LUAD 治療的一個(gè)有希望的靶點(diǎn)。非編碼 RNA (ncRNA)，包括 microRNA (miRNA)、長(zhǎng)鏈非編碼 RNA (lncRNA) 和環(huán)狀 RNA (circRNA)，在人類癌癥的發(fā)病機(jī)制中發(fā)揮著許多重要的調(diào)節(jié)功能，顯示了全面了解其機(jī)制的必要性肺癌的發(fā)生。盡管如此，對(duì)許多已知轉(zhuǎn)錄本和信使 RNA (mRNA) 的研究已經(jīng)產(chǎn)生了新的信息。 ncRNA 中的未知生物標(biāo)志物以及與未知 ncRNA 和 mRNA 的系統(tǒng)和全面的相互關(guān)系可能會(huì)為 LUAD 的生物學(xué)提供進(jìn)一步的見解。在此，我們鑒定了一組新的 ncRNA，包括 miRNA、lncRNA 和 circRNA，并使用嚴(yán)格的生物信息學(xué)流程獲得了 LSC 和 ALDH-H1975 LUAD 腫瘤細(xì)胞 (LTC) 中 ncRNA 和 mRNA 的差異表達(dá)模式。通過(guò)對(duì)已識(shí)別景觀的薈萃分析，構(gòu)建了新的競(jìng)爭(zhēng)性內(nèi)源性 RNA (ceRNA) 網(wǎng)絡(luò)，以揭示調(diào)節(jié) LSC 和 LTC 標(biāo)志的潛在分子機(jī)制。本研究總結(jié)了新型 ncRNA 以及差異表達(dá)的 ncRNA 在 LSC 和 LTC 活性中的基本作用。此外，該研究還為未來(lái)識(shí)別 LUAD 中的診斷、治療和預(yù)后生物標(biāo)志物提供了綜合資源。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國(guó)際數(shù)據(jù)庫(kù)描述：

ALDH+ H1975 lung adenocarcinoma stem cells (LSCs) are a rare cell population identified in lung adenocarcinoma (LUAD). LSCs can self-renew, drive tumor initiation, growth, metastasis, and recurrence and are also the predominant cause of poor prognosis due to their intrinsic resistance to drugs and chemotherapy. Consequently, LSCs are a promising target for LUAD therapy. Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), exert many significant regulatory functions in the pathogenesis of human cancers, showing the necessity for a comprehensive understanding of the mechanisms that underlie lung carcinogenesis. Nonetheless, research on many known transcripts and messenger RNAs (mRNAs) has already generated new information. Unknown biomarkers in ncRNAs and systematic and comprehensive interrelation with unknown ncRNAs and mRNAs may provide further insights into the biology of LUAD. Herein, a set of novel ncRNAs that include miRNAs, lncRNAs, and circRNAs were identified, and differentially expressed patterns of ncRNAs and mRNAs in LSCs and ALDH-H1975 LUAD tumor cells (LTCs) were obtained using stringent bioinformatics pipelines. Through a meta-analysis of the identified landscapes, novel competitive endogenous RNA (ceRNA) networks were constructed to reveal the potential molecular mechanisms that regulate the hallmarks of LSCs and LTCs. This study presents a summary of novel ncRNAs and the fundamental roles of differentially expressed ncRNAs implicated in the activity of LSCs and LTCs. In addition, the study also provides a comprehensive resource for the future identification of diagnostic, therapeutic, and prognostic biomarkers in LUAD.