【佳學(xué)基因檢測(cè)】惡性黑色素瘤中骨橋蛋白亞型和整合素的基因表達(dá)模式

國(guó)內(nèi)正規(guī)腫瘤基因檢測(cè)機(jī)構(gòu)關(guān)鍵點(diǎn)

深究基因檢測(cè)機(jī)構(gòu)自我培訓(xùn)教材看到《Pathol Oncol Res》在.?2022 Aug 24;28:1610608.發(fā)表了一篇題目為《惡性黑色素瘤中骨橋蛋白亞型和整合素的基因表達(dá)模式》腫瘤靶向藥物治療基因檢測(cè)臨床研究文章。該研究由Krisztina Jámbor,?Viktória Koroknai,?Tímea Kiss,?István Szász,?Péter Pikó,?Margit Balázs等完成。促進(jìn)了腫瘤的正確治療與個(gè)性化用藥的發(fā)展，進(jìn)一步強(qiáng)調(diào)了基因信息檢測(cè)與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究?jī)?nèi)容關(guān)鍵詞：

基因表達(dá),整合素,黑色素瘤進(jìn)展,骨橋蛋白,骨橋蛋白剪接變體

腫瘤靶向治療基因檢測(cè)臨床應(yīng)用結(jié)果

骨橋蛋白 (OPN) 是一種多功能糖蛋白，在生理上與不同類型的整合素相互作用。它被認(rèn)為是某些腫瘤類型中可能的預(yù)后生物標(biāo)志物；然而，存在各種剪接異構(gòu)體，尚未在黑色素瘤中進(jìn)行研究。我們旨在確定五種 OPN 同種型的相對(duì)表達(dá)模式，并闡明剪接變體在黑色素瘤中的預(yù)后意義。我們還旨在研究八種整合素在同一腫瘤中的表達(dá)模式。基因表達(dá)分析顯示，與原發(fā)灶相比，轉(zhuǎn)移性腫瘤中 OPNa、OPNb 和 OPNc 的相對(duì)表達(dá)顯著高于原發(fā)灶（p < 0.01），而 OPN4 和 OPN5 在兩者中的表達(dá)均較低。與表面擴(kuò)散亞型相比，更具侵襲性的結(jié)節(jié)性黑色素瘤具有更高的表達(dá)水平（p ≤ 0.05）。八種測(cè)試整合素的相對(duì)表達(dá)較低，僅在結(jié)節(jié)性黑色素瘤中可檢測(cè)到 ITGB3 的表達(dá)（Medianlog2 = 1.274）。 Breslow 厚度與 OPNc 變體的表達(dá)呈正相關(guān)，因此較厚的腫瘤（> 4 mm）具有顯著更高的表達(dá)（p ≤ 0.05）。 Breslow厚度與OPN4的表達(dá)呈負(fù)相關(guān)，與ITGA2的表達(dá)相似。 OPNc也與轉(zhuǎn)移的存在呈顯著正相關(guān)。我們的數(shù)據(jù)表明，OPNa、OPNb，尤其是 OPNc 的高表達(dá)和 OPN4 和 ITGA2 的低表達(dá)與腫瘤進(jìn)展的晚期和黑色素瘤的不良預(yù)后相關(guān)。整合素；黑色素瘤進(jìn)展；骨橋蛋白；骨橋蛋白剪接變體。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國(guó)際數(shù)據(jù)庫(kù)描述：

Osteopontin (OPN) is a multifunctional glycoprotein that physiologically interacts with different types of integrins. It is considered to be a possible prognostic biomarker in certain tumor types; however, various splicing isoforms exist, which have not been investigated in melanoma. We aimed to define the relative expression pattern of five?OPN?isoforms and clarify the prognostic significance of the splice variants in melanoma. We also aimed to investigate the expression pattern of eight integrins in the same tumors. Gene expression analyses revealed that the relative expression of?OPNa, OPNb, and?OPNc?is significantly higher in metastatic tumors compared to primary lesions (p?< 0.01), whereas the expression of?OPN4?and?OPN5?was low in both. The more aggressive nodular melanomas had higher expression levels compared to the superficial spreading subtype (p?≤ 0.05). The relative expression of the eight tested integrins was low, with only the expression of?ITGB3?being detectable in nodular melanoma (Medianlog2?= 1.274). A positive correlation was found between Breslow thickness and the expression of?OPNc?variant, whereby thicker tumors (>4 mm) had significantly higher expression (p?≤ 0.05). The Breslow thickness was negatively correlated with the expression of?OPN4, and similarly with?ITGA2.?OPNc?also exhibited significant positive correlation with the presence of metastasis. Our data show that high expression of?OPNa,?OPNb, and especially?OPNc?and low expression of?OPN4?and?ITGA2?are associated with an advanced stage of tumor progression and poor prognosis in melanoma.gene expression; integrins; melanoma progression; osteopontin; osteopontin splice variants.